Facility user policies:
- COVID-19 related policies and phased reopening plans
User Policies_CUNY ASRC Imaging_COVID-19 phased opening_e4
- Reservation schedule before 11/01/2021
To make reservations, we are using this Google Sheet instead of Badger until 10/31/2021. Reservation request, cancellation, modification must be made via email to BOTH facility staff with subject title “Imaging Instrument Reservation/Cancellation”, cancellation should be made no later than 5:00pm prior to reservation date. Please do not come to facility until you receive confirmation email. Staff will add confirmed reservations to Google Sheet (users have no permission to edit the sheet).
- Reservation schedule after 11/01/2021
Starting from 11/01/2021, we will be back using Badger system to make instrument reservations. The Google sheet will be discontinued after that day. For experienced users, who do not need assistance from facility staff, please make reservations directly in Badger system following the facility user policies. For non-experienced users, who need staff assistance or training purposes, please contact the facility staff to confirm availability before making reservations in Badger. New users must finish all the user registration steps and are able to login Badger with their accounts before scheduling training.
CUNY-ASRC-Imaging-Facility_Badger SOP
- General user policies
- General
- Safety
- Reservation
- Off hours policy
- Acknowledgement
CUNY-ASRC-Imaging-Facility_General User Policies
- Computer use policy:
CryoEM Data Processing Computer
User Policies_CUNY ASRC Imaging_cryoEM data processing
Image Reconstruction Computer (Window OS with Amira/Avizo and other softwares)
User Policies_CUNY ASRC Imaging_Image Reconstruction Computer
Badger instruction:
CUNY-ASRC-Imaging-Facility_Badger SOP
Badger Dashboard: http://cuny1.badgerlms.com/badger/DashboardIS-1.html
Instrument instructions and documents:
-
Titan Halo 300kV TEM
SOP_Titan Halo 300kV TEM Procedure_2021 e2
SOP_Cryoholder controller & puming station
serialEM-note02-Single particle data collection – stitching MMM – MR e3
serialEM-note03-setup-LD-with-mP-and-nP e2
serialEM-note04-Multiple Record Setup Dialog e2
-
Titan Themis 200kV S/TEM
200 kV Titan Themis Alignment_2021
-
Tecnai Spirit 120kV TEM
SOP_Tecnai Spirit TWIN 120 kV TEM
-
Helios Nanolab 660 FIB-SEM
FEI Helios NanoLab 660 Dualbeam FIB-SEM
TEM Lamella Sample Preparation
2D Image Stack Using Auto Slice & View
-
WITec Raman microscope
WITec Raman Microscope alpha300R
- Leica STED
STED_Sample preparation guide-AppLetter_EN
- FEI Vitrobot
- Fischione NanoClean
Fischione NanoCleam M1070 Procedure
- Leica EM ACE600 coater
Leica EM ACE600 Coater Procedure
Forms:
Other resources:
General
- Visotor information (building access, travel by public transportation and by car, parking facilities) (https://asrc.gc.cuny.edu/about/visitor-information/)
Nanoscience
- ASRC Sensor CAT (https://asrc.gc.cuny.edu/about/sensor-cat/)
Structural Biology
- Online class “Getting Started in cryoEM” by Grant Jensen at Caltech (http://www.cryo-em-course.caltech.edu/)
- Lectures on structural biology software including cryo-EM packages hosted by SBGrid consortium (https://www.youtube.com/playlist?list=PLVetO1F9gm_oa–j37yrjzJ4yVJYzPVN5)
- Lecture series on EM given at the MRC Laboratory of Molecular Biology (ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/EM-course)
- Lectures from workshops organized by the National Resource for Automated Molecular Microscopy (NRAMM) (http://nramm.nysbc.org/workshops-and-courses/?lcp_page0=2#lcp_instance_0)