Mapping and modeling the impact of protein biochemical variation on growth rate phenotype Individual proteins can be expressed, purified, and exquisitely characterized in terms of their biochemical and biophysical parameters in vitro. However, the quantitative relationship between these parameters and complex phenotypes like growth remains mysterious. For example, what values of protein abundance, thermal stability

Dynamic RNA-protein interactions in A-to-I editing and biomolecular condensates in non- coding RNA pathways We employ integrative structural biology, combining solution NMR with complementary techniques and molecular dynamics to study the dynamics of RNAs and RNA-protein interactions in RNA processing pathways, including alternative splicing regulation and non- coding RNA pathways. The structural understanding is a

Catching Rare Events in Action with Weighted Ensemble MD Rare biological and chemical events often lie beyond the reach of conventional simulations. The weighted ensemble (WE) path sampling strategy overcomes this barrier, extending accessible timescales by orders of magnitude while maintaining rigorous kinetics. By directly simulating pathways and stepwise rates, WE reveals molecular mechanisms in