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ASRC-CCNY Seminar Series in Biochemistry, Biophysics and Biodesign: Melanie Cocco, University of California Irvine

Join us for the fall semester ASRC-CCNY Seminar Series in Biochemistry, Biophysics and Biodesign every Wednesday at noon!

Melanie Cocco
Associate Professor, Molecular Biology & BiochemistryAssociate Professor, Dept. of Pharmaceutical SciencesUniversity of California, Irvine

Protein Dynamics Determined from NMR Hydrogen Exchange Measurements and Hemoglobin Stability with Voxelotor


Abstract    NMR spectroscopy provides a measure of protein dynamics in solution that is useful in understanding the relative stabilities of mutants, drug-bound states, or homologous proteins.  Hydrogen-deuterium exchange reactions occur during a timescale specific to protein folding events and can provide details on local unfolding and/or global stability.  Here we present three studies of hydrogen exchange in DNA-binding protein domains (DBDs).  The cytidine repressor (CytR) is a bacterial sensor that regulates the production of enzymes needed for nucleic acid production.  CytR has been identified as a target in the treatment of cholera and urinary tract infections.  We compare the structure and stability of the CytR protein to the lactose repressor (LacR).  Hydrogen exchange for another system, the DBD of the p53 transcription regulator reveals how the structure of this protein can be stabilized by rescue mutations that restore function of this important anti-cancer protein.  A third system, a thermally stable archaeal bypass polymerase Dbh is shown by hydrogen exchange to maintain structural features in proximity to the catalytic domain at high temperatures.      Hemoglobin is the last system to be discussed.  Voxelotor (aka Oxbryta) is a new drug developed to treat sickle cell disease by binding the hemoglobin tetramer and maintaining the protein in a stable soluble conformation.  We examine the effect of this drug on the natural degradation of hemoglobin in solution.

Download Melanie Cocco Seminar Flyer

Download the updated Fall 2023 Seminar Schedule

Dr. Cocco will be giving this talk in person at the ASRC Main Auditorium. For non-CUNY attendees: advance registration is required; please contact Jennifer Chow at jchow@gc.cuny.edu no later than Monday, Oct. 16 for entry to the ASRC.

THIS SEMINAR MAY ALSO BE VIEWED REMOTELY VIA ZOOM: Meeting ID:  966 7763 1144Passcode:  asrc-ccny

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Event Information

October 18, 2023
11:30 am - 1:00 pm
Advanced Science Research Center (ASRC)
85 St. Nicholas Terrace
New York, NY 10031 United States
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(212) 413-3300
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