Join us for the fall semester ASRC-CCNY Seminar Series in Biochemistry, Biophysics and Biodesign every Wednesday at noon! The final speaker of this fall series, Rachel Narehood Austin, Professor in the Dept. of Chemistry at Barnard College, Columbia University, will present a talk titled, “Exploring the structure and function of alkane monooxygenase (AlkB): a key metalloenzyme in the carbon cycle.”
ABSTRACT Alkane hydroxylase (AlkB) is a membrane-spanning non-heme diiron enzyme with a histidine-rich coordination site, first identified 50 years ago, responsible for catalyzing the hydroxylation of the majority of medium-to-long straight chain alkanes in the environment. AlkB catalyzes the hydroxylation of terminal alkanes, the epoxidation of alkenes, and, in selected cases, the desaturation of alkanes. Despite its importance in the global carbon cycle, relatively little is known about this large and widely-distributed class of
hydroxylases. We have studied the reaction mechanism and substrate specificity of AlkB from Pseudomonas putida GPo1, the marine organism Alcanivorax borkumensis AP1, Dietzea cinnamea, and Fontimonas thermophila. We have recently solved the cryo-EM structure of AlkB from Fontimonas thermophila. Integrating information from targeted mutations,
reactivity studies, and the three-dimensional structure has shed light on structure-function relationships in this important class of integral membrane monooxygneases, while raising additional questions to be solved.
This seminar will be presented in the ASRC Auditorium and broadcast via Zoom, with snacks and refreshments to start at 11:30am in the cafe.
To join via Zoom:
Meeting ID: 966 7763 1144
Passcode: asrc-ccny
For more information about this hybrid event, please contact:
Lauren Gohara
lgohara@ccny.cuny.edu
(212) 650-8803