- Associate Professor, Structural Biology Initiative
- Associate Professor of Chemistry & Biochemistry, The City College of New York
Daniel Keedy, Ph.D.
Professor Keedy begin his appointment at CUNY as an Assistant Professor in January 2018 and was promoted to Associate Professor in September 2025. He is affiliated with the Structural Biology Initiative at the CUNY Advanced Science Research Center (ASRC); the Department of Chemistry and Biochemistry at the City College of New York (CCNY); and the Ph.D. Programs in Biochemistry, Biology, and Chemistry at the CUNY Graduate Center.
Prior to his appointment at CUNY, Professor Keedy was an A.P. Giannini Postdoctoral Fellow in Bioengineering and Therapeutic Sciences at the University of California, San Francisco working with Professor James Fraser. He earned his Ph.D. in Biochemistry and Structural Biology & Biophysics from Duke University, after receiving his B.A. in Biochemistry & Molecular Biology from Rhodes College.
His research on protein structural flexibility, temperature-dependent X-ray crystallography, and allosteric regulation has been supported by funding from the National Institutes of Health, including the National Institute of General Medical Sciences and the National Institute on Aging, and from the Department of Energy. Professor Keedy has also received a Cottrell Scholar Award and a CCNY CCAPP STEM Teacher of the Year Award.
About the Keedy Lab
The Keedy Lab combines computation and experiments to reveal alternative protein conformations and explore how they underlie dynamic functions such as catalysis, ligand binding, and allosteric regulation.
Research Interests
Professor Keedy develops experimental and computational methods to control proteins by biasing toward specific conformations that underlie functions such as allostery, ligand binding, and catalysis. His work reveals new opportunities to modulate the activities of therapeutic targets such as tyrosine phosphatases with small molecules and protein engineering, and also offers insights into more general evolutionary processes that led to functional diversity in the human proteome.
Publications
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Guerrero L, Ebrahim A, Riley BT, Kim SH, Bishop AC, Wu J, Han YN, Tautz L, Keedy DA. Three STEPs Forward: A Trio of Unexpected Structures of PTPN5. Proteins: Structure, Function, and Bioinformatics. 2025 |
Woods VA*, Sharma S*, Lemberikman AM, Keedy DA. Orchestrating function: concerted dynamics, allostery, and catalysis in protein tyrosine phosphatases. Current Opinion in Structural Biology. 2025. |
Wu J, Baranowski MR, Aleshin AE, Isiorho EA, Lambert LJ, De Backer LJS, Han YN, Das R, Sheffler DJ, Bobkov AA, Lemberikman AM, Keedy DA, Cosford NDP, Tautz L. Fragment Screening Identifies Novel Allosteric Binders and Binding Sites in the VHR (DUSP3) Phosphatase. ACS Omega. 2025. |
Wankowicz SA, Ravikumar A, Sharma S, Riley BT, Raju A, Hogan D, van den Bedem H, Keedy DA, Fraser JS. Automated multiconformer model building for X-ray crystallography and cryo-EM. eLife. 2024. |
Woods VA, Abzalimov RR, Keedy DA. Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS. Protein Science. 2024. 33(6):e5024. |
Guerrero L*, Ebrahim A*, Riley BT, Kim M, Huang Q, Finke AD, Keedy DA (*contributed equally). Pushed to extremes: distinct effects of high temperature versus pressure on the structure of STEP. Communications Biology. 2024. 7(1):59. |
Sharma S, Mehlman (Skaist) T, Sagabala RS, Boivin B, Keedy DA. High-resolution double vision of the allosteric phosphatase PTP1B. Acta Cryst F. 2024. 80(Pt 1):1-12. |
Mehlman T. S., Biel J. T., Azeem S. M., Nelson E. R., Hossain S., Dunnett L., Paterson N. G., Douangamath A., Talon R., Axford D., Orins H., von Delft F., Keedy D. A. Room-temperature crystallography reveals altered binding of small-molecule fragments to PTP1B. eLife 2023 12:e84632 |
