Amédée des Georges, Ph.D.

  • Assistant Professor of Chemistry, Structural Biology Initiative
  • Assistant Professor of Chemistry & Biochemistry, The City College of New York

Profiles: Google Scholar, ORCID

Amédée des Georges, Ph.D. is a structural biologist with particular expertise in single-particle cryo-electron microscopy. He uses the ability of single-particle cryo-electron microscopy to sort electron microscopy images into different sub-populations in order to obtain high resolution structures of samples conformationally or compositionally heterogeneous, such as the mammalian translation initiation complex or the largest known membrane channel called the ryanodine receptor. He established a research group exploring with cryo-electron microscopy the regulatory mechanisms of such large protein and RNA complexes.

Des Georges received his B.S. and M.S. in Biochemistry from Université Pierre and Marie Curie in Paris before obtaining his Ph.D. degree from the University of Cambridge in 2008 for his work with Linda Amos at the MRC-Laboratory of Molecular Biology. He then joined the lab of Joachim Frank at Columbia University as a postdoctoral researcher. In addition of being a core faculty member of the ASRC Structural Biology Initiative, des Georges is a tenure-track assistant professor at the City College Department of Chemistry and Biochemistry.

Des Georges was appointed to the ASRC in August 2015.

Research Interests

Structural and functional study of the regulation of large macromolecular complexes using cryo-electron microscopy.

Publications

H. Bansia, C. Catalano, Z. Melville, Y. Guo, A.R. Marks, A. des Georges. Investigating gating mechanisms of ion channels using temperature-resolved cryoEM. Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1690 - 1694. DOI: 10.1017/S1431927621006206

A. Dashti, G. Mashayekhi, M. Shekhar, D. Ben Hail, S. Salah, P. Schwander, A. des Georges, A. Singharoy, J. Frank, A. Ourmazd. Retrieving functional pathways of biomolecules from single-particle snapshots. Nature Communications, 2020, 11, no. 1 : 1-14.

T. C. Marcink, T. Wang, A. des Georges, M. Porotto, A. Moscona. Human parainfluenza virus fusion complex glycoproteins imaged in action on authentic viral surfaces. PLoS pathogens, 2020, 16, no. 9: e1008883.

T.C. Marcink, E. Yariv, K. Rybkina, V. Más, F. T. Bovier, A. des Georges, A. L. Greninger et al. Hijacking the Fusion Complex of Human Parainfluenza Virus as an Antiviral Strategy. Mbio, 2020, 11, no. 1.

A.H. Nguyen, A. R. B. Thomsen, T. J. Cahill, R. Huang, L. Huang, T. Marcink, O. B. Clarke, A. des Georges et al. Structure of an endosomal signaling GPCR–G protein–β-arrestin megacomplex. Nature structural & molecular biology, 2019, 26, no. 12: 1123-1131.

L.C. Montemiglio, C. Testi, P. Ceci, E. Falvo, M. Pitea, C. Savino, A. Arcovito, G. Peruzzi, P. Baiocco, F. Mancia, A. Boffi, A. des Georges, B. Vallone. Cryo-EM structure of the human ferritin-transferrin receptor 1 complex. Nat Commun. 2019, 10(1), 1121. DOI: 10.1038/s41467-019-09098-w.

A. Dashti, D.B. Hail, G. Mashayekhi, P. Schwander, A. des Georges, J. Frank, A. Ourmazd (co-corresponding author). Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots. 2019, bioRxiv: 291922 (pre-print)

J. Chase, A. Catalano, A. J. Noble, E. T. Eng, P. DB Olinares, K. Molloy, D. Pakotiprapha, A. des Georges, et al. Mechanisms of opening and closing of the bacterial replicative helicase. Elife, 2018, 7: e41140.

L. Calisti, M.C. Trabuco, A. Boffi, C. Testi, L.C. Montemiglio, A. des Georges, et al. Engineered ferritin for lanthanide binding. PLoS ONE, 2018, 13, 8, e0201859. DOI: 10.1371/journal.pone.0201859

A. J. Noble, V. P. Dandey, H. Wei, J. Brasch, J. Chase, P. Acharya, G. Scarpin M. Rapp, E. T. Eg, W. J. Rice, A. Cheng, C. Negro, L. Shapiro, P.D. Kwong, D. Jeruzalmi, A. des Georges, C. Potter, B. Carragher. Routine single particle CryoEM sample and grid characterization by tomographyeLife, 2018, 7, e34257. DOI: 10.7554/eLife.34257

G. Santulli, D. Lewis, A. des Georges, A. R. Marks, J. Frank. Ryanodine receptor structure and function in health and disease. Membrane Protein Complexes: Structure and Function, 2018, 329-352. DOI: 10.1007/978-981-10-7757-9_11

Y. Hashem, A. des Georges, J. Fu, S.N. Buss, F. Jossinet, A. Jobe, Q. Zhang, H.Y. Liao, R.A. Grassucci, C. Bajaj, E. Westhof, S. Madison-Antenucci, J. Frank. High-resolution cryo-electron microscopy structure of the Trypanosoma brucei ribosome. Series in Structural Biology Single-Particle Cryo-Electron Microscopy, pp. 456-462. DOI: 10.1142/9789813234864_0047

A. des Georges, O.B. Clarke, R. Zalk, Q. Yuan, K.J. Condon, R.A. Grassucci, W.A. Hendrickson, A.R. Marks, J. Frank. Structural Basis for Gating and Activation of RyR1. Series in Structural Biology Single-Particle Cryo-Electron Microscopy, pp. 497-515. DOI: 10.1142/9789813234864_0051

Kazemi M, Sorzano COS, Carazo JM, Georges AD,Abrishami V, Vargas J. ENRICH: A fast method to improvethe quality of flexible macromolecular reconstructions. Prog Biophys Mol Biol. 2021 Jan 12;. doi:10.1016/j.pbiomolbio.2021.01.001

V. de Turris, M.C. Trabuco, G. Peruzzi, A. Boffi, C. Testi, B. Vallone, L.C. Montemiglio, A. Des Georges, L. Calisti, I. Benni, A. Bonamore, P. Baiocco. Humanized archaeal ferritin as a tool for cell targeted delivery. Nanoscale, Volume 9, Issue 2, Pages 647-655. DOI: 10.1039/C6NR07129E

A. des Georges, O.B. Clarke, R. Zalk, Q. Yuan, K.J. Condon, R.A. Grassucci, W.A. Hendrickson, A.R. Marks, J. Frank. Structural Basis for Gating and Activation of RyR1. Cell. 2016 Sep 22;167(1):145-157.e17. doi: 10.1016/j.cell.2016.08.075.

S. Belh, A. Manandhar, G. Huffman, K. Ng, A. Chowdhury, M. Patel, N. Yehya, A. des Georges, S. Loverde, D. Eisele. Counterion interactions in supramolecular self-assembly. Abstracts of Papers of the American Chemical Society, Volume 252.

R. Zalk, O.B. Clarke, A. des Georges, R.A. Grassucci, S. Reiken, F. Mancia, W.A. Hendrickson, J. Frank, A.R. Marks. Structure of a mammalian ryanodine receptorNature 517, n. 7532, 2015: 44-49.

des Georges A, Dhote V, Kuhn L, Hellen CU, Pestova TV, Frank J, Hashem Y. Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Nature. 2015 Sep 24;525(7570):491-5. doi: 10.1038/nature14891. Epub 2015 Sep 7. PubMed PMID: 26344199; PubMed Central PMCID: PMC4719162.

des Georges, A., Hashem, Y., Unbehaun, A., Grassucci, R. A., Taylor, D., Hellen, C. U., Pestova, T. V., Frank, J. Structure of the mammalian ribosomal pre-termination complex associated with eRF1*eRF3*GDPNP. Oxford Academic, Nucleic Acids Research, Volume 42, Issue 5, 1 March 2014, Pages 3409–3418.

Y. Hashem, A. des Georges, V. Dhote, R. Langlois, H.Y. Liao, R.A. Grassucci, T. V. Pestova, C. U. T. Hellen, J. Frank. Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit. Nature Volume 503, Pages 539–543. DOI: 10.1038/nature12658

A. des Georges, Y. Hashem, S.N. Buss, F. Jossinet, Q. Zhang, H.Y. Liao, J. Fu, A. Jobe, R.A. Grassucci, R. Langlois, C. Bajaj, E. Westhof, S. Madison-Antenucci, J. Frank. High-resolution Cryo-EM Structure of the Trypanosoma brucei Ribosome: A Case Study. Computational Methods for Three-Dimensional Microscopy Reconstruction pp 97-132. DOI: 10.1007/978-1-4614-9521-5_5

Jobe A., Hashem Y., des Georges A., Fu J., Buss S. N., Jossinet F., Zhang Q., Liao H. Y., Grassucci B., Bajaj C., Madison-Antenucci S., Westhof E., Frank J. High-resolution cryo-EM structure of the Trypanosoma brucei 80S: a unique eukaryotic ribosome. Journal of Biomolecular Structure and Dynamics, Volume 31, 2013, Issue sup1. doi: 10.1080/07391102.2013.786465

Hashem, Y., Des Georges, A., Dhote, V., Langlois, R., Liao, H.Y., Grassucci, R.A., Hellen, C.U.T., Pestova, T.V., Frank, J. Structure of the mammalian ribosomal 43S preinitiation complex bound to the scanning factor DHX29. Cell.com,  Volume 153, Issue 5, P1108-1119. doi.org/10.1016/j.cell.2013.04.036

Y. Hashem, A. Des Georges, J. Fu, S.N. Buss, F. Jossinet, A. Jobe, Q. Zhang, H.Y. Liao, R.A. Grassucci, C. Bajaj, E. Westhof, S. Madison-Antenucci, J. Frank. High-resolution cryo-electron microscopy structure of the Trypanosoma brucei ribosome. Nature Volume 494, Pages 385–389 (2013) DOI: 10.1038/nature11872

Schaap IA, Eghiaian F., des Georges A., Veigel C. Effect of envelope proteins on the mechanical properties of influenza virus. Journal of Biological Chemistry, Volume 287, Issue 49, P41078-41088. doi.org/10.1074/jbc.m112.412726
F. Eghiaian, I.A. Schaap, A. Des Georges, J.J. Skehel, C. Veigel. The Influenza Virus Mechanical Properties Are Dominated By Its Lipid Envelope. RID:0219141157558-5 Biophysical Journal, Volume 96, Issue 3, Supplement 1, 15A.

des Georges, A. Regulation of Tubulin dynamics by the +Tip tracking protein Mal3 https://doi.org/10.17863/CAM.475

A. des Georges, M. Katsuki, D. R Drummond, M. Osei, R. A. Cross, L. A. Amos. Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice. Nature Structural & Molecular Biology volume 15, pages 1102–1108 (2008)

M Katsuki, M Osei, DR Drummond, A des Georges, LA Amos, RA Cross, Towards In Vitro Reconstitution of Regulated S. pombe Microtubule Dynamics: The Synergy of Mal3 and Tea2. Molecular Biology of the Cell, 2006

headshot: amedee de georges

Phone Number

212.413.3232