Zeiss LSM 880 Airyscan Fast Live Cell

The Zeiss LSM 880 Airyscan Fast Live Cell is a unique confocal that provides high resolution, sensitivity and speed that enables gentle live cell imaging with super resolution. Airyscan delivers super resolution of 140 nm XY, and 4-8 times sensitivity. The FAST module increases speed 4 times up to 100 fps, which increase productivity when performing quantitative imaging. Temperature, CO2, humidity controlled incubator is optimized for time lapsing live imaging.

Features

• Airyscan to achieve 1.7x resolution (140nm XY, 400nm Z) and 4-8x sensitivity
• FAST model for 4x speed up to 100fps
• Axio Observer inverted platform with motorized XYZ stage
• Definite focus 2 prevent focus drift over time
• Piezo with 500um travel range for extremely fast Z stacks
• Tokai-Hit stagetop incubation chamber
• Zen Black software with the modules of Z stacks, timelapse,  tiling, multi position, co localization, FRAP, super resolution, deconvoltuion, 3D processing.

Laser Lines

• Argon:  458, 488, 514 nm
• Diode: 405 nm
• DPSS: 561 nm
• HeNe: 633 nm.

Objectives

• EC Plan Neofluor 10x/0.3 NA
• Plan Apo 20x/0.8
• Plan Apo 40x/1.2 NA Water or Type W Oil
• Plan Apo 63x/1.4 NA Oil DIC

Detectors

• 2 PMT’s
• 1 GaAsP (2x sensitivity)
• 1 Airyscan super resolution detector
• 1 Transmitted light detector

Applications

• Super resolution confocal imaging of fixed or live samples, photoactivation, or photoconversion in live cells or organisms
• Capturing fast events such as Ca2+ imaging
• FRAP
• FRET

Contacts

• Ye He, Ph.D.
  Director, Live Imaging and Bioenergetics Facility
  Co-Director, MALDI-TOF MS Imaging Facility
  Research Associate Professor, Neuroscience Initiative
  yhe1@gc.cuny.edu