PippinHT

The Pippin systems allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. Pippin HT excels at high-throughput applications and also works with smaller sample amounts. Pippin HT uses disposable gel cassettes that are precast with agarose to perform DNA size selection in ranges between 100bp up to 1.5kb and collect the desired DNA fragments based on user-input values. High-Pass protocols are available for collecting all larger DNA above a fragment size.

Features

• Generates higher-quality libraries for improved sequence informatics
• Fragment sizes and ranges are reproducibly collected, providing consistent results, run-to-run
• Flexible programming allows collection of multiple size ranges, or up to 24 collections of the same fragment range
• The Pippin HT agarose cassettes available in the Core and the size ranges in which samples can be sized:
  • 75% from 6-10 kb, high –pass
  • 75% from 15-20 kb, high –pass
  • 75% from 30-40 kb, high -pass
  • 5% from 250 bp up to 1.5 kb
  • 2% from 100 bp up to 600 bp
  • 3% from 100 bp to 250 bp

Contacts

• Jia Liu, Ph.D.
  Director, Epigenetics Facility
  Director, Rodent Behavioral Analysis Suite
  Research Associate Professor, Neuroscience Initiative
  jliu1@gc.cuny.edu