The MassARRAY System is a non-fluorescent detection platform utilizing mass spectrometry to discriminate methylated vs. non-methylated DNA; includes software, reagents, and MALDI-TOF mass spectrometry for detection and quantification.
EpiTYPER biochemistry starts with bisulfite treatment of genomic DNA, followed by PCR amplification of target regions. The reverse primers contain a T7 promoter tag. Next, in vitro RNA transcription is performed, followed by base-specific RNA cleavage. Finally, the cleavage products are analyzed using MALDI-TOF mass spectrometry (MassARRAY Analyzer). The methylated and non-methylated cytosine residues in the original genomic DNA are easily distinguished using EpiTYPER Software.
- Interrogates 10s-100s of samples and CpG sites in amplicons from 200 – 600 bp and detect down to 5% differences in methylation.
- The EpiTYPER technology includes online assay design software, robust biochemistry steps, and precise detection of reaction products using highly quantitative mass spectrometry.
- Can also be used for other nucleic acid analysis methods, including SNP and somatic mutation detection.
- 96 well Chips