MassARRAY System with Epityper DNA Methylation Analysis

The MassARRAY System is a non-fluorescent detection platform utilizing mass spectrometry to discriminate methylated vs. non-methylated DNA; includes software, reagents, and MALDI-TOF mass spectrometry for detection and quantification.

EpiTYPER biochemistry starts with bisulfite treatment of genomic DNA, followed by PCR amplification of target regions. The reverse primers contain a T7 promoter tag. Next, in vitro RNA transcription is performed, followed by base-specific RNA cleavage. Finally, the cleavage products are analyzed using MALDI-TOF mass spectrometry (MassARRAY Analyzer). The methylated and non-methylated cytosine residues in the original genomic DNA are easily distinguished using EpiTYPER Software.

Features

• Interrogates 10s-100s of samples and CpG sites in amplicons from 200 – 600 bp and detect down to 5% differences in methylation.
• The EpiTYPER technology includes online assay design software, robust biochemistry steps, and precise detection of reaction products using highly quantitative mass spectrometry.
• Can also be used for other nucleic acid analysis methods, including SNP and somatic mutation detection.

Available Formats

• 96 well Chips

Contacts

• Jia Liu, Ph.D.
  Director, Epigenetics Facility
  Director, Rodent Behavioral Analysis Suite
  Research Associate Professor, Neuroscience Initiative
  jliu1@gc.cuny.edu