10X Visium CytAssist

The 10x Genomics Visium CytAssist is a compact benchtop instrument that streamlines spatial transcriptomics by transferring transcriptomic probes from standard glass tissue slides (FFPE, Fresh Frozen, or Fixed Frozen) to Visium specialized slides. It enables precise alignment of tissue sections, allows pre-screening, and supports both gene and protein expression analysis with 6.5×6.5 mm capture areas. Visium CytAssist allows spatial transcriptomics profiling experiment by sectioning the tissue onto a standard glass slide. After deparaffinization (for FFPE samples), staining (H&E or IF), and imaging the sections, they are de-crosslinked and hybridized with transcriptomic probes to inform presence of a target RNA. Following probe hybridization, Visium CytAssist facilitates transfer of these transcriptomic probes from the glass slide to a Capture Area on the Visium slide. The Visium platform delivers unbiased, whole transcriptome spatial gene expression analysis at single cell scale with unmatched spatial data quality and offers a flexible assay portfolio to study a broad range of tissues.

Features

• Simplify sample handling with facilitated transfer of transcriptomic probes from standard slides onto the Visium Capture Area
• Enabling users to start with tissue on standard glass slides, allowing input tissue to be prepared, stained, and imaged using standard histology workflows.
• Expanded compatibility with FFPE and/or FF samples.

Key Supported Applications

• Visium HD WT (the whole transcriptome probe-based WT Panel gene expression assay, which is only available for human, rhesus, and mouse tissue samples)
• Visium HD 3’ (the 3’ poly(A) capture-based gene expression assay, which is species agnostic)

Contacts

• Jia Liu, Ph.D.
  Director, Epigenetics Facility
  Director, Rodent Behavioral Analysis Suite
  Research Associate Professor, Neuroscience Initiative
  [email protected]