Digitizing Proteoform Biology with Single Molecule & Single Cell Mass Spectrometry
Since the completion of the Human Genome Project, much has been made of the need to bridge the gap from genes and traits. As a key nexus for the many interacting ‘-omes’ (genome,
transcriptome, proteome, metabolome, etc.), the proteome should offer a tight link between genotype and phenotype. Proteoforms, or all of the precise molecular forms of a protein, capture all sources of variability in protein composition (i.e., SNPs, isoforms, posttranslational modifications), and thus provide crucial insights into regulation and function. Now, “single ion” mass spectrometry is poised to convert genes to proteoform signatures at a far faster rate. Recently we developed proteoform imaging mass spectrometry (PiMS), with individual ion mass spectrometry. This platform has been extended now to single-cell Proteoform imaging Mass Spectrometry (scPiMS), boosting cell processing rates by >20-fold in the field while detecting proteoforms from single cells.
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For any questions, please contact Hyacinth Camillieri at hcamillieri@gc.cuny.edu