Dynamic RNA-protein interactions in A-to-I editing and biomolecular condensates in non- coding RNA pathways
We employ integrative structural biology, combining solution NMR with complementary techniques and molecular dynamics to study the dynamics of RNAs and RNA-protein interactions in RNA processing pathways, including alternative splicing regulation and non- coding RNA pathways. The structural understanding is a starting point to identify small molecule inhibitors that modulate these pathways for novel therapeutic approaches. Starting from a brief outline of our work and approaches in splicing regulation, two studies will be presented: (1) We discovered extensive dynamics associated with A-to-I hyper-edited dsRNAs, which exhibit unique conformational features. These features are specifically associated with hyper-editing and are specifically recognized by inosine binding proteins such as Endonuclease V. (2) We are dissecting the molecular principles of biomolecular condensates that are implicated in the separation of small non-coding RNA pathways in Drososphila, combining NMR, biophysical techniques and cell microscopy.
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For any questions, contact Hyacinth Camillieri at hcamillieri@gc.cuny.edu.